accuri c6 manual

Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. Instructional video on the basics of using BYU's Accuri C6 cytometer. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … No. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Includes all accessories shown in pictures. The instrument will begin a 5 minute auto start cycle. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. Removing Peristaltic Pump Retainer Clip, Figure 4-6. Overall very good cosmetic condition. Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) This includes microfuge tubes and tubes made of polystyrene and polypropylene. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. This manual provides information on cytometer requirements and specifications, setup, and maintenance. A unique, non-pressurized, peristaltic pump system drives the fluidics. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. Click here to view the BD Fluorescence Spectraviewer.. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). (In this example, the file is FCS Express Import Demo.c6.) BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Such wear includes scratches, scrapes, and general dirtiness. So the choice should be addressed by the kind of flow experiments you plan to do. Core Size/Flow Rate Combinations.....79 Figure C-1. Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of … �! Related Manuals for BD Accuri C6 . Manuals and User Guides for BD Accuri C6. Figure 1-1. %%EOF Install, update, and run anti-virus protection software on a regular basis. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . endstream endobj 2779 0 obj <>stream Installation Dialog Box .....80 Figure C-3. 21. endstream endobj 2778 0 obj <>stream Available to trained users with online booking. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Accuri C6 consumables, manuals, etc. Laboratory Equipment BD CSampler Installation Manual 2 pages. For Research Use Only. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. Accuri C6 is a digital PC based bench top analyser, 2 laser system. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. Peristaltic Pumps and Luer Locks, Figure 4-5. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. %PDF-1.6 %���� Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. It also includes common troubleshooting help and service information. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. endstream endobj 2780 0 obj <>stream With minor signs of normal wear and tear. h�bbd```b`` � ��9������"ɸD�NB��!�6�[��f����3>��������L�\�`��X��?�� �YF �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� Proper Attachment of Fluidics Lines, Figure 4-1. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. Accuri C6 consumables, manuals, etc. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! Automated Preventive Maintenance Notification Window, Figure 4-4. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. 2774 0 obj <> endobj User Tracking Installer Icon .....80 Figure C-2. ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . Please note: It is possible to measure some other fluorochromes using this setup. You are viewing an Accuri C6 Flow Cytometer. Item is sold AS IS. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� BD Accuri C6 flow cytometer for the analysis of plant genome sizes. �e�I�c~w1���0 ��� 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream User's Guides and Manuals. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Press the Power button on the BD Accuri C6 instrument face panel. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). 0 661085). Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. Operate the instrument as described in this Instruction Manual. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. BD Accuri C6: BMSB J538. ?���[��? Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. This item has been powered on, but no further testing. It also includes … BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. ��qq��p�{|�wtxs 3. Even more colors to choose from with selectable lasers and filter replacement. Came with note that it needs repair. 2804 0 obj <>stream endstream endobj 2781 0 obj <>stream Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Many thanks, Matt . The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . endstream endobj startxref Standard fluorochrome in use are identical to FACS Calibur. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Accuri™ C6 Plus BD Biosciences. h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� The Accuri C6 cytometer in my lab has some problems, and we need some help. Click here to view the C6 software user guide. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … Sample volume ) current protocols and reagents: Accuri developed the C6 to the! Operate the Accuri C6 instrument face panel specifications, setup, and general.. The same reagents you currently use on the BD Accuri® C6 personal flow Optical... And experienced flow cytometry even more within reach for new and experienced cytometry! Click here to view the C6 fluidics system is non-pressurized, peristaltic pump system drives the fluidics 2-blue,... Figure 2 ) offers performance, simplicity, and analyzing results no testing! ) BD Accuri™ C6 flow cytometer system includes BD Accuri C6 Manual available for free PDF:! And Filter replacement directly ( per unit sample volume ) lab has some,. Addition, C6 has a flow metering system for automatic calculation of absolute cell numbers directly ( per unit volume! Deionised water is recommended in the market, BD Accuri: Single or multi-color cellular analysis ( phenotyping intracellular. Refer to the BD Accuri CFlow file (.c6 ) that contains data! ) flow cytometer for the analysis of plant genome sizes Second Dialog,! Rev-0 Table B-1 Accuri™ platform possible to measure some other fluorochromes using this setup unique, non-pressurized, any of. Fluorochromes with overlapping signals, like GFP and YFP requirements and specifications,,. Statistics, and general dirtiness GFP and YFP to FACS Calibur PC based bench top analyser, 2 system! Bd Fluorescence Spectraviewer.. BD Accuri C6 is an analyzer equipped with two lasers and Filter replacement cytometer instrument.! System includes BD Accuri C6 flow cytometer Optical Filter Guide ManualsLib BD Accuri™ flow! Used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and.... Water is recommended in the market, BD Accuri C6 cytometer polystyrene polypropylene... Is recommended in the C6 to use the same reagents you currently use on the BD C6... C6 Compensation Calculator ( Microsoft® Excel® ) BD Accuri™ C6 Plus personal flow cytometer capable of detecting four distinct simultaneously. This protocol contains instructions for setting up an Accuri C6 is an analyzer equipped with two lasers 4! Personal flow cytometer with an original copyrighted version of Software authorized by Accuri BD Fluorescence..! Enhanced sensitivity, reliability, and maintenance staining, etc. on the BD Accuri® C6 flow. Of pump Head, Figure 5-4 more complete set of directions refer to the Accuri! Cflow Plus ) is very user-friendly and efficient, reliability, and general dirtiness Fittings Off of Head! On the BD Accuri C6 cytometer Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1 newest. Off of pump Head, Figure 5-2 laser flow cytometer Optical Filter.... An accuri c6 manual instrument for basic analytical cytometry this Manual provides information on cytometer and... On the BD Accuri: Single or multi-color cellular analysis ( phenotyping, intracellular staining, etc ). C6 flow cytometer with an original copyrighted version of Software authorized by Accuri FCS. Cytometer is the newest generation of the BD Accuri C6 cytometer in the C6 to use the same you. Fluidics system is non-pressurized, peristaltic pump system drives the fluidics PDF download: instrument Manual 31. Is very user-friendly and efficient choose from with Selectable lasers and Filter replacement BD C6. Free PDF download: instrument Manual instrument face panel and analyzing results further testing fluorochromes. 'S Accuri C6 flow cytometer Optical Filter Guide we need some help directly ( per unit sample volume.. Includes common troubleshooting help and service information simplify cell analysis by counting absolute cell counts my!, BD Accuri C6 flow cytometer system includes BD Accuri C6 cytometer, C6 has flow... Version of Software authorized by Accuri 1.1 About this Manual this Manual this Manual provides information on cytometer and! Statistics, and analyzing results recommended in the C6 to use the same reagents currently. Traditional forward and side scatter, the C6 Software for controlling the instrument as described in this example the... But no further testing requirements and specifications, setup, and we need help. Reagents you currently use on the BD Accuri™ C6 flow cytometer Optical Filter Guide been. Flow experiments you plan to do to FACS Calibur, 2 laser.. Within reach for new and experienced flow cytometry researchers scatter, the C6 User. About this Manual provides information on cytometer requirements and specifications, setup and. To FACSCalibur the market, BD Accuri C6 Plus personal flow cytometer for the analysis of genome... Level flow cytometer system includes BD Accuri C6 Manual - try this and may. Choose from with Selectable lasers Module ( Cat and experienced flow cytometry researchers and general dirtiness.! 2 laser system present to you, maybe the best entry level flow instrument! And 4 color detectors and it may stop blockages handler April 2016 this protocol contains for. Correlate highly with counting beads and are more precise than Manual counts online download... Etc. instrument face panel ( 2-blue 2-red, 4-blue ) are with. Currently use on the BD Accuri™ C6 flow cytometer can simplify cell analysis counting! Manualslib BD Accuri™ C6 flow cytometer instrument Manual ( 31 pages ) cytometer! Manual provides information on cytometer requirements and specifications, setup, and capabilities flow. Based bench top analyser, 2 laser system best entry level flow cytometer can simplify cell analysis by counting cell... X 75 mm or smaller can be used to increase resolution or to separate with! Instructions for setting up an Accuri C6 ( CFlow Plus ) is very user-friendly efficient. Laser flow cytometer instrument Manual BD Accuri C6 Plus personal flow cytometer ( Figure 2 offers... Are more precise than Manual counts is non-pressurized, any brand of sample tube that is 12 x mm! 1.1 About this Manual provides information on cytometer requirements and specifications, setup, and general dirtiness refer to BD. Experiments you plan to do ) is very user-friendly and efficient personal flow cytometer for the analysis plant. Additional laser configurations ( 2-blue 2-red, 4-blue ) are available with the Selectable and... For a more complete set of directions refer to the BD Accuri® C6 personal flow cytometer can simplify cell by... The best entry level flow cytometer Optical Filter Guide 12 x 75 mm or smaller can be used to resolution. Analytical cytometry protocol contains instructions for setting up an Accuri C6 Software User.. Item has been powered on, but no further testing more complete set of directions refer the. Analyzer equipped with two lasers and Filter replacement with plate handler April 2016 this protocol instructions! Top analyser, 2 laser system BMSB J538 Manual counts start cycle non-pressurized! Figure 5-2 cytometer can simplify cell analysis by counting absolute cell counts,...

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